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植物脅迫測量套件

簡要描述:Y(II)或ΔF/Fm’ 或 (Fm’ – Fs )/Fm’) 是經受時間考驗的光適應測量參數,比Fv/Fm對更多類型的植物脅迫更加敏感。已有的大量證據表明Fv/Fm對許多種植物脅迫和健康植物的光系統(tǒng)II的測量十分出色,而Y(II)或光量子產額則可測量實際光照下光適應環(huán)境和生理狀況的光系統(tǒng)II的效率。

  • 產品型號:PSK
  • 廠商性質:生產廠家
  • 更新時間:2024-10-15
  • 訪  問  量:781

詳細介紹

  應用
 
  Y(II)或ΔF/Fm’ 或 (Fm’ – Fs )/Fm’) 是經受時間考驗的光適應測量參數,比Fv/Fm對更多類型的植物脅迫更加敏感。已有的大量證據表明Fv/Fm對許多種植物脅迫和健康植物的光系統(tǒng)II的測量十分出色,而Y(II)或光量子產額則可測量實際光照下光適應環(huán)境和生理狀況的光系統(tǒng)II的效率。
 
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        原理
 
  采用調制飽和脈沖原理,測量植物的葉綠素熒光,測量參數包括植物的光量子產額Y(II)及相對電子傳遞速率ETR,最大光化學效率Fv/Fm,同時還可測量PAR、葉溫、相對濕度和葉片吸光率等環(huán)境參數。
 
  特點
 
  葉片吸光率測量:提供葉片吸收測量及隨環(huán)境變化導致的葉片吸收變化。根據Eichelman (2004) 葉片吸收在健康植物的變化范圍在0.7~0.9 之間。因此,為獲得準確的ETR或“J”,Y(II)測量儀提供了一個可靠的測量方法,
 
  Fv/Fm測量單元:用于暗適應測量。
 
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  先進的PAR葉夾:采用底部葉夾打開裝置,防止測量時誤操作打開葉夾。對傳感器進行余弦校正,確保葉片相對測量光的角度不變。
 
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  Fm’校正:對于具有高光照強度歷史的植物,*關閉光反應中心是一個問題,Y(II)測量儀使用Loriaux &Genty 2013的方法進行Fm’ 校正,確??梢詼y得準確的Fm’ 。
 
  自動調制光設定:快速準確自動的調整合適的調制光強,避免人工操作的誤差。
 
  先進算法避免飽和脈沖NPQ:采用25ms內8點的平均值確定Fm、Fm’、Fo、Fs,消除飽和脈沖NPQ的影響和電子噪音。
 
  更精確的葉溫測量:采用非接觸式紅外測量,測量精度可達±0.5℃。
 
  直接測量相對濕度:含有測量氣體交換使用的固態(tài)傳感器,可測量相對濕度。
 
  降低葉片遮擋的設計:傾斜的角度減少對葉片的遮擋,可以測量擬南芥等小葉。
 
  系統(tǒng)組成
 
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標配:
  Y(II)光量子產額測量儀,Fv/Fm測量儀及10個暗適應葉夾,2個電池,2個充電器,一個便攜箱,文件U盤。
 
  技術指標
 
  測量參數
 
  Y(II)或ΔF/Fm‘、ETR、PAR、Tleaf、相對濕度、Fms或Fm’、Fs、α(葉片吸收率)、FV/FM、FV/FO,FO, FM, FV。
 
  監(jiān)測模式:允許長時間監(jiān)測
 
  技術參數
 
  Y(II): 光適應測量, 穩(wěn)態(tài)光合作用下的環(huán)境光
 
  光源
 
  飽和脈沖: LED白光源,使用PAR葉夾時可達7000μmols
 
  調制光:紅光,LED 660nm,具有690nm窄通過濾器。
 
  光化光源:環(huán)境光
 
  檢測方法:脈沖調制法
 
  PAR:測量400-700nm,余弦校正 ±2umols
 
  Fv/Fm:暗適應測量
 
  光源:LED紅光飽和光閃,可達6000umols;
 
  調制光:660nmLED 紅光,690nm濾波器
 
  調制光可以根據實際測量自動調節(jié)到合適的強度,減少手動調節(jié)誤差,
 
  相對濕度:0%~100%,±2%。
 
  檢測器&過濾器:具有700~750nm帶通過濾的PIN光電二極管
 
  可選配三腳架。
 
  顯示:132 X 30 pixel 液晶顯示屏
 
  取樣速率:1~10000點/秒自動切換。
 
  測量時間:最短3s或也可設置長期監(jiān)測模式
 
  存儲空間:2GB
 
  輸出:USB下載數據,用Excel查看,無需安裝其他專用軟件
 
  供電:USB鋰離子電池(普通充電寶),可用8小時
 
  尺寸:便攜箱尺寸為14”x 11”x 6”,儀器為9’’長
 
  質量:Y(II) 測量儀0.45 kg
 
  Fv/Fm測量儀0.36 kg.
 
  加便攜箱和附件總重1.95 kg.
 
  工作溫度:0℃ ~ 50℃
 
  產地
 
  美國
 
  文獻
 
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  Adams WW III, Demmig-Adams B. (1994) Carotenoid composition and down regulation of Photosystem II in three conifer species during the winter. Physiol Plant 92: 451-458
 
  Ball MC. (1994) The role of photoinhibition during seedling establishment at low temperatures. In: Baker NR. And Bowyer JR. (eds) Photoinhibition of Photosynthesis. From Molecular Mechanisms to the Field, pp365-3376 Bios Scientific Publishers, Oxford
 
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  Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, The Netherlands, page 627-628 Burke J. (2007) Evaluation of Source Leaf Responses to Water-Deficit Stresses in Cotton Using a Novel Stress Bioassay, Plant Physiology, Jan. 2007, Vol 143, pp108-121
 
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  Cazzaniga S, Osto L.D., Kong S-G., Wada M., Bassi R., (2013) “Interaction between avoidance of photon absorption, excess energy dissipation and zeaxanthin synthesis against photo oxidative stress in Arabidopsis”, The Plant Journal, Volume 76, Issue 4, pages568–579, November 2013 DOI: 10.1111/tpj.12314
 
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  Adams WW III, Demmig-Adams B., Vernhoeven AS., and Barker DH., (1995) Photoinhibition during winter stress – Involvement of sustained xanthophyll cycle-dependent energy-dissipation. Aust J. Plant Physiol 22: 261-276 Journal of Experimental Botany, 55(403):1607-1621
 
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  all’Osto L, Cazzaniga S, Wada M, Bassi R. (2014) On the origin of a slowly reversible fluorescence decay component in the Arabidopsis npq4 mutant. Phil. Trans. R. Soc. B 369: 20130221.htt://dx.doi.org/10.1098/rstb.2013.0221
 
  da Silva J. A. & Arrabaca M.C. (2008).Physiologia Plantarum Volume 121 Issue 3, Pages 409 – 420 2008
 
  Eichelman H., Oja V., Rasulov B., Padu E., Bichele I., Pettai H., Niinemets O., Laisk A. (2004) Development of Leaf Photosynthetic Parameters in Betual pendula Roth Leaves: Correlation with Photosystem I Density, Plant Biology 6 (2004):307-318
 
  Eyodogan F., Oz M. T. (2007) Effect of salinity on antioxidant responses of chickpea seedlings. Acta Physiol Plant (2007) 29:485-493
 
  Flexas 1999 – “Water stress induces different levels of photosynthesis and electron transport rate regulation in grapevines”J. FLEXAS, J. M. ESCALONA & H. MEDRANO Plant, Cell & Environment Volume 22 Issue 1 Page 39-48, January 1999
 
  Flexas 2000 – “Steady-State and Maximum Chlorophyll Fluorescence Responses to Water Stress In Grape Vine Leaves: A New Remote Sensing System”, J. Flexas, MJ Briantais, Z Cerovic, H Medrano, I Moya, Remote Sensing Environment 73:283-270 Physiologia Plantarum, Volume 114, Number 2, February 2002 , pp. 231-240(10)
 
  Gonias E. D. Oosterhuis D.M., Bibi A.C. & Brown R.S. (2003) YIELD, GROWTH AND PHYSIOLOGY OF TRIMAX TM TREATED COTTON, Summaries of Arkansas Cotton Research 2003
 
  Hendrickson L., Furbank R., & Chow (2004) A simple alternative approach to assessing the fate of absorbed Light energy using chlorophyll fluorescence. Photosynthesis Research 82: 73-81
 
  Kramer D. M., Johnson G., Kiirats O., Edwards G. (2004) New fluorescence parameters for determination of QA redox state and excitation energy fluxes. Photosynthesis Research 79: 209-218
 
  Krause G.H., Weis E. (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. 5, 139-157.
 
  Krupa Z., Oquist G., and Huner N., (1993) The effects of cadmium on photosynthesis of Phaseolus vulgaris – a fluorescence analysis. Physiol Plant 88, 626-630
 
  D Edwards GE and Baker NR (1993) Can CO2 assimilation in maize leaves be predicted accurately from chlorophyll fluorescence analysis? Photosynth Res 37: 89–102
 
  Laisk A and Loreto F (1996) Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluorescence. Ribulose-1,5-bisphosphate carboxylase / oxygenase specificity factor, dark respiration in the light, excitation distribution between photosystems, alternative electron transport rate, and mesophyll diffusion resistance. Plant Physiol 110: 903–912
 
  Photosynthesis in the water-stressed C grass is mainly limited by stomata with both rapidly and slowly imposed water deficits. Flexas (2002) Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO2 assimilation and stomatal conductance during water-stress in C plants Flexas J., Escalona J. M., Evain S., Gulías J., Moya I., Charles Barry Osmond C.B., and Medrano H. 4 Setaria sphacelata
 
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  Loriaux S.D., R.A Burns,Welles J.M., McDermitt D.K. Genty B. (2006) “Determination of Maximal Chlorophyll Fluorescence Using A Multiphase Single Flash of Sub-Saturating Intensity”. Abstract # P13011 August 1996.
 
  American Society of Plant Biologists Annual Meetings, Boston MA LORIAUX S.D, AVENSON T.J., WELLES J.M., MCDERMITT D.K., ECKLES R. D., RIENSCHE B. & GENTY B. (2013) Closing in on maximum yield of chlorophyll fluorescence using a single multiphase flash of sub-saturating intensity Plant, Cell and Environment (2013) 36, 1755–1770 doi: 10.1111/pce.12115
 
  Maai E., Shimada S., Yamada M.,, Sugiyama T., Miyake H., and Taniguchi M., (2011) The avoidance and aggregative movements of mesophyll chloroplasts in C4 monocots in response to blue light and abscisic acid Journal of Experimental Botany, Vol. 62, No. 9, pp. 3213–3221, 2011, doi:10.1093/jxb/err008 Advance Access publication 21 February, 2011
 
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  Nedbal L. Whitmarsh J. (2004) Chlorophyll Fluorescence Imaging of Leaves and Fruits From Chapter 14, “Chlorophyll a Fluorescence a Signature of Photosynthesis”, edited by George Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, TheNetherlands, page 389 -407
 
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  Strasser R.J, Tsimilli-Michael M., and Srivastava A. (2004) - Analysis of Chlorophyll a Fluorescence Transient. From Chapter 12, “Chlorophyll a Fluorescence a Signature of Photosynthesis”, edited by George Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, The Netherlands, page 340 Tripathy BC, Bhatia B., Mohanty P., (1981) Inactivation of chloroplast photosynthetic electron transport activity by Ni ++. Biochim Biophys Acta 638:217-224
 
  Vredenberg W.,  Kay J. and Russotti R. (2013) The instrumental implementation of a routine for quantitative analysis of photochemical-induced variable chlorophyll fluorescence in leaves: Properties and prospects. ISPR conference in St. Louis, Poster mail: wim.vredenberg@wur.nl mail: ?iv ák M., Bresti M., Olšovská K., Slamka P.(2008) Performance index as a sensitive indicator of water stress in PLANT SOIL ENVIRON., , 2008 (4): 133–139
 
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